Engineering of a Chimeric Template Triggers RNase H-Based Isothermal Amplification Approach for Sensitive Detection of Pathogen RNA

Author:

Li Yong1,Zhang Xin1,Liao Yu1,Shi Chao2,Wang Ye3,Mu Xiaofeng3,Xie Yingqiu4,Ma Cuiping1ORCID

Affiliation:

1. Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, Qingdao Key Laboratory of Nucleic Acid Rapid Detection, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China

2. Qingdao Navid Biotechnology Co., Ltd, Qingdao JianMa Gene Technology Co., Ltd, Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, the Affiliated Hospital of Qingdao University, College of Life Sciences, Qingdao University, Qingdao 266071, China

3. Clinical Laboratory, Qingdao Central Hospital, The Second Affiliated Hospital of Medical College of Qingdao University, Qingdao 266042, China

4. Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana 010000, Kazakhstan

Funder

Natural Science Foundation of Shandong Province

Taishan Industrial Experts Program

Publisher

American Chemical Society (ACS)

Subject

Analytical Chemistry

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