Site-Specific and Quantitative N-Glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-Resolution Two-Dimensional Gel Electrophoresis and Mass Spectrometry-Reference-Cited by-同舟云学术

Site-Specific and Quantitative N-Glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-Resolution Two-Dimensional Gel Electrophoresis and Mass Spectrometry

Published:2020-03-26 Issue:8 Volume:92 Page:5695-5700
ISSN:0003-2700
Container-title:Analytical Chemistry
language:en
Short-container-title:Anal. Chem.

Author:

Li Xiaoyu¹²^ORCID,He Qing³,Yu Zixiang²,Xie Yuping²,Zhao Xinyuan²^ORCID,Huang Yi²,Wang Yongzhong⁴,Ke Xiao³,Qian Xiaohong²,Dong Junxing¹⁵,Ying Wantao¹²^ORCID

Affiliation:

1. College of Life Science and Bioengineering, Beijing University of Technology, No. 100, Pingleyuan, Chaoyang District, Beijing, 100124, China

2. State Key Laboratory of Proteomics, National Center for Protein Sciences (Beijing), Beijing Proteome Research Center, Beijing Institute of Lifeomics, No. 38, Life Park Road, Changping District, Beijing, 102206, China

3. Therapeutic Proteins Key Laboratory of Sichuan Province, Chengdu Kanghong Pharmaceutical Group Co., Ltd., No. 36, Shuxi road, Jinniu District, Chengdu, Sichuan 610036, China

4. Suqiao Biologics (Suzhou) Co., Ltd, No. 188, Dongping Street, Industrial Park, Suzhou, Jiangsu 215123, China

5. Beijing Institute of Radiation Medicine, Beijing, 100850, China

Funder

Ministry of Science and Technology of the People's Republic of China

National Natural Science Foundation of China

Innovation Foundation of Medicine

Publisher

American Chemical Society (ACS)

Subject

Analytical Chemistry

Link

https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.0c00592