Development and Validation of a New Robust Detection Method for Low-Content DNA Using ΔΔCq-Based Real-Time PCR with Optimized Standard Plasmids as a Control Sample-Reference-Cited by-同舟云学术

Development and Validation of a New Robust Detection Method for Low-Content DNA Using ΔΔCq-Based Real-Time PCR with Optimized Standard Plasmids as a Control Sample

Published:2022-10-07 Issue:41 Volume:94 Page:14475-14483
ISSN:0003-2700
Container-title:Analytical Chemistry
language:en
Short-container-title:Anal. Chem.

Author:

Soga Keisuke¹^ORCID,Nakamura Kosuke¹,Egi Tomohiro²,Narushima Jumpei¹,Yoshiba Satoko¹,Kishine Masahiro³,Mano Junichi³,Kitta Kazumi³^ORCID,Takabatake Reona³^ORCID,Shibata Norihito¹^ORCID,Kondo Kazunari¹

Affiliation:

1. Division of Biochemistry, National Institute of Health Sciences, 3-25-26, Tonomachi, Kawasaki-ku, Kawasaki-shi210-9501, Kanagawa, Japan

2. Food and Agricultural Materials Inspection Center: Saitama Shintoshin National Government Building, Kensato Building 2-1, Shintoshin, Chuo-ku, Saitama-Shi330-9731, Saitama, Japan

3. National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba305-8642, Ibaraki, Japan

Funder

Japan Society for the Promotion of Science

Ministry of Health, Labour and Welfare

Consumer Affairs Agency, Government of Japan

Publisher

American Chemical Society (ACS)

Subject

Analytical Chemistry

Link

https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.2c03680

Reference38 articles.

1. Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods

2. Real-time PCR detection chemistry

3. Variable Sensitivity of SARS-CoV-2 Molecular Detection in European Expert Laboratories: External Quality Assessment, June and July 2020

4. External Quality Assessment for Molecular Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in Clinical Laboratories

5. A study of quality assessment in SARS-CoV-2 pathogen nucleic acid amplification tests performance; from the results of external quality assessment survey of clinical laboratories in the Tokyo Metropolitan Government external quality assessment program in 2020

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