Novel Multidimensional Liquid Chromatography Workflow with In-Loop Enzymatic Digests of Multiple Heart-Cuts for Fast and Flexible Characterization of Biotherapeutic Protein Variants-Reference-Cited by-同舟云学术

Novel Multidimensional Liquid Chromatography Workflow with In-Loop Enzymatic Digests of Multiple Heart-Cuts for Fast and Flexible Characterization of Biotherapeutic Protein Variants

Published:2023-02-06 Issue:7 Volume:95 Page:3629-3637
ISSN:0003-2700
Container-title:Analytical Chemistry
language:en
Short-container-title:Anal. Chem.

Author:

Mayr Kilian¹,Weindl Thomas¹^ORCID,Gärtner Achim¹,Camperi Julien²,Maetzke Thomas³,Förster Markus⁴,Nachtigall Thomas⁴,Steiner Frank⁴,Vogt Annette¹,Hosp Fabian¹^ORCID,Mølhøj Michael¹

Affiliation:

1. Large Molecule Research, Roche Innovation Center Munich, Roche Pharma Research and Early Development, Roche Diagnostics GmbH, 82377 Penzberg, Germany

2. Cell Therapy Engineering and Development, Genentech, 1 DNA Way, South San Francisco, California, 94080, United States

3. HPLConsult GmbH, Bergmattenweg 16, CH-4148 Pfeffingen, Switzerland

4. Thermo Fisher Scientific GmbH, Im Steingrund 4-6, 63303 Dreieich, Germany

Publisher

American Chemical Society (ACS)

Subject

Analytical Chemistry

Link

https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.2c04467

Reference23 articles.

1. Direct Identification of Rituximab Main Isoforms and Subunit Analysis by Online Selective Comprehensive Two-Dimensional Liquid Chromatography–Mass Spectrometry

2. Three Dimensional Liquid Chromatography Coupling Ion Exchange Chromatography/Hydrophobic Interaction Chromatography/Reverse Phase Chromatography for Effective Protein Separation in Top-Down Proteomics