High-Throughput, Quantitative Enzyme Kinetic Analysis in Microdroplets Using Stroboscopic Epifluorescence Imaging
Author:
Affiliation:
1. Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Vladimir Prelog Weg 1, 8093 Zürich, Switzerland
Funder
Eidgenössische Technische Hochschule Zürich
National Research Foundation of Korea
Schweizerische Nationalfonds zur Förderung der Wissenschaftlichen Forschung
Publisher
American Chemical Society (ACS)
Subject
Analytical Chemistry
Link
https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.5b00766
Reference38 articles.
1. Droplet microfluidics for high-throughput biological assays
2. Millisecond Kinetics on a Microfluidic Chip Using Nanoliters of Reagents
3. Droplet-Based Microfluidic Flow Injection System with Large-Scale Concentration Gradient by a Single Nanoliter-Scale Injection for Enzyme Inhibition Assay
4. Enzyme Kinetic Measurements Using a Droplet-Based Microfluidic System with a Concentration Gradient
5. Measuring Rapid Enzymatic Kinetics by Electrochemical Method in Droplet-Based Microfluidic Devices with Pneumatic Valves
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