1. Kabanov, A. V.; Felgner, P. L.; Seymour, L. W., Eds.Self-assemblingcomplexes for gene delivery. From laboratory to clinical trial. John Wiley & Sons: New York, 1998.
2. PEO-b-PMANa was synthesized by anionic polymerization as described before (Kabanov, A. V.; Bronich, T. K.; Kabanov, V. A.; Yu, K.; Eisenberg, A.Macromolecules1996,29, 6797−6802). The segment lengths were 176 and 186 repeating units for PEO and PMANa, respectively. The following surfactants were used: dodecyltrimethylammonium bromide (DDTAB), tetradecyltrimethylammonium bromide (TDTAB), cetyltrimethylammonium bromide (CTAB), dodecylpyridinium chloride (C12PyCl), and cetylpyridinium bromide (C16PyBr). The complexes were prepared by mixing PEO-b-PMANa and surfactant aqueous solutions at room temperature in the absence of added electrolyte. In all cases, the final concentration of the PEO-b-PMANa carboxylate groups was 0.6 mM, the [surfactant]/[carboxylate group] ratio varied from 0.1 to 1.0, pH 8.4 to 8.6. No additional mechanical agitation commonly used for vesicle preparation was applied.
3. Particle ζ-potential was measured as previously described (Bronich, T. K.; Kabanov, A. V.; Kabanov, V. A.; Yu, K.; Eisenberg, A.Macromolecules1997,30, 3519−3525).
4. Micellization in Block Polyelectrolyte Solutions. 3. Static Light Scattering Characterization