Droplet Cas12a Assay Enables DNA Quantification from Unamplified Samples at the Single-Molecule Level

Author:

Yue Huahua12,Shu Bowen34ORCID,Tian Tian1,Xiong Erhu1ORCID,Huang Mengqi1,Zhu Debin5,Sun Jian678,Liu Qing9,Wang Shichan10,Li Yirong10,Zhou Xiaoming1ORCID

Affiliation:

1. School of Life Sciences, South China Normal University, Guangzhou 510631, China

2. College of Biophotonics, South China Normal University, Guangzhou 510631, China

3. Dermatology Hospital, Southern Medical University, Guangzhou 510091, China

4. Department of Laboratory Medicine, Guangzhou First People’s Hospital, School of Medicine, South China University of Technology, Guangzhou 510180, China

5. Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, School of Chemistry, South China Normal University, Guangzhou 510006, China

6. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China

7. National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China

8. Research Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou 510642, China

9. Department of Molecular Diagnostics, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510700, China

10. Department of Laboratory Medicine, Zhongnan Hospital, Wuhan University, Wuhan 430071, China

Funder

Ministry of Education of the People's Republic of China

National Natural Science Foundation of China

Guangdong Province

Guangdong Basic and Applied Basic Research Foundation

Publisher

American Chemical Society (ACS)

Subject

Mechanical Engineering,Condensed Matter Physics,General Materials Science,General Chemistry,Bioengineering

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