Effect of the Competing Ligand on Ni-NTA/Histag Strength Revealed by Click Chemistry-Based Force Spectroscopy
Author:
Affiliation:
1. State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210023, P. R. China
Funder
Natural Science Foundation of Jiangsu Province
Fundamental Research Funds for the Central Universities
National Natural Science Foundation of China
Publisher
American Chemical Society (ACS)
Subject
General Materials Science,Physical and Theoretical Chemistry
Link
https://pubs.acs.org/doi/pdf/10.1021/acs.jpclett.3c00813
Reference55 articles.
1. Purification of Recombinant Proteins with Metal Chelate Adsorbent
2. How Strong Is the Coordination Bond between a Histidine Tag and Ni - Nitrilotriacetate? An Experiment of Mechanochemistry on Single Molecules
3. Recognition Force Spectroscopy Studies of the NTA-His6 Bond
4. Reversible Unfolding of Individual Titin Immunoglobulin Domains by AFM
5. Navigating the Folding Energy Landscape of Green Fluorescent Protein
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