Positive Identification of DNA Cleavage by CRISPR-Cas9 Using Pyrene Excimer Fluorescence to Detect a Subnanometer Structural Change
Author:
Affiliation:
1. Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea
2. Department of Chemistry, Chung-Ang University, Seoul 06974, Republic of Korea
Publisher
American Chemical Society (ACS)
Subject
General Materials Science,Physical and Theoretical Chemistry
Link
https://pubs.acs.org/doi/pdf/10.1021/acs.jpclett.9b01913
Reference32 articles.
1. Development and Applications of CRISPR-Cas9 for Genome Engineering
2. A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
3. DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
4. Conformational control of DNA target cleavage by CRISPR–Cas9
5. Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3′-terminal segment of guide RNA
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