Unraveling the Mechanism of a LOV Domain Optogenetic Sensor: A Glutamine Lever Induces Unfolding of the Jα Helix

Author:

Iuliano James N.1ORCID,Collado Jinnette Tolentino1,Gil Agnieszka A.2ORCID,Ravindran Pavithran T.2,Lukacs Andras34,Shin SeungYoun1,Woroniecka Helena A.1,Adamczyk Katrin3,Aramini James M.5,Edupuganti Uthama R.56,Hall Christopher R.3,Greetham Gregory M.7,Sazanovich Igor V.7,Clark Ian P.7,Daryaee Taraneh1,Toettcher Jared E.2ORCID,French Jarrod B.18ORCID,Gardner Kevin H.5910ORCID,Simmerling Carlos L.1ORCID,Meech Stephen R.3ORCID,Tonge Peter J.1ORCID

Affiliation:

1. Department of Chemistry, Stony Brook University, New York, 11794, United States

2. Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, United States

3. School of Chemistry, University of East Anglia, Norwich, NR4 7TJ, United Kingdom

4. Department of Biophysics, Medical School, University of Pecs, Szigeti út 12, 7624 Pecs, Hungary

5. Structural Biology Initiative, CUNY Advanced Science Research Center, 85 St. Nicholas Terrace, New York, New York 10031, United States

6. Ph.D. Program in Biochemistry, CUNY Graduate Center, New York, New York, United States

7. Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, OX11 0QX, United Kingdom

8. Hormel Institute, University of Minnesota, Austin, Minnesota 55912, United States

9. Ph.D. Programs in Biochemistry, Biology, and Chemistry, CUNY Graduate Center, New York, New York, United States

10. Department of Chemistry and Biochemistry, City College of New York, New York, New York, United States

Funder

Engineering and Physical Sciences Research Council

Directorate for Biological Sciences

National Institute of Biomedical Imaging and Bioengineering

National Institute of General Medical Sciences

Bolyai Janos Research Fellow

Publisher

American Chemical Society (ACS)

Subject

Molecular Medicine,General Medicine,Biochemistry

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