R150A Mutant of F TraI Relaxase Domain: Reduced Affinity and Specificity for Single-Stranded DNA and Altered Fluorescence Anisotropy of a Bound Labeled Oligonucleotide-Reference-Cited by-同舟云学术

R150A Mutant of F TraI Relaxase Domain: Reduced Affinity and Specificity for Single-Stranded DNA and Altered Fluorescence Anisotropy of a Bound Labeled Oligonucleotide

Published:2002-04-27 Issue:20 Volume:41 Page:6460-6468
ISSN:0006-2960
Container-title:Biochemistry
language:en
Short-container-title:Biochemistry

Author:

Harley Matthew J.¹,Toptygin Dmitri¹,Troxler Thomas¹,Schildbach Joel F.¹

Affiliation:

1. Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, and Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104

Publisher

American Chemical Society (ACS)

Subject

Biochemistry

Link

https://pubs.acs.org/doi/pdf/10.1021/bi011969i

Reference38 articles.

1. Firth, N., Ippen-Ihler, K., and Skurray, R. A. (1996) inEscherichia coli and Salmonella, 2nd ed. (Neidhardt, F. C., Ed.) pp 2377−2401, ASM Press, Washington, DC.

2. Analysis of the sequence and gene products of the transfer region of the F sex factor

3. Evidence that DNA helicase I and oriT site-specific nicking are both functions of the F TraI protein

4. Endonuclease activity of Escherichia coli DNA helicase I directed against the transfer origin of the F factor.

5. F Plasmid Conjugative DNA Transfer

Cited by 23 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Live-cell microscopy or fluorescence anisotropy with budded baculoviruses—which way to go with measuring ligand binding to M ₄ muscarinic receptors?;Open Biology;2022-06

2. Live-cell microscopy or fluorescence anisotropy with budded baculoviruses - which way to go with measuring ligand binding to M4 muscarinic receptors?;2021-12-23

3. Conjugative DNA Transfer Is Enhanced by Plasmid R1 Partitioning Proteins;Frontiers in Molecular Biosciences;2016-07-19

4. Completing the specificity swap: Single-stranded DNA recognition by F and R100 TraI relaxase domains;Plasmid;2015-07

5. Protein and DNA Effectors Control the TraI Conjugative Helicase of Plasmid R1;Journal of Bacteriology;2009-11-15