Ancestral Sequence Reconstruction Identifies Structural Changes Underlying the Evolution of Ideonella sakaiensis PETase and Variants with Improved Stability and Activity

Author:

Joho Yvonne12,Vongsouthi Vanessa2ORCID,Spence Matthew A.2,Ton Jennifer2,Gomez Chloe2,Tan Li Lynn2ORCID,Kaczmarski Joe A.23,Caputo Alessandro T.1,Royan Santana1,Jackson Colin J.243ORCID,Ardevol Albert15

Affiliation:

1. Manufacturing, Commonwealth Scientific and Industrial Research Organisation, Clayton, Victoria 3168, Australia

2. Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia

3. ARC Centre of Excellence for Innovations in Synthetic Biology, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia

4. ARC Centre of Excellence for Innovations in Peptide & Protein Science, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia

5. CSIRO Synthetic Biology Future Science Platform, GPO Box 1700, Canberra, ACT 2601, Australia

Funder

Australian Research Council

Commonwealth Scientific and Industrial Research Organisation

Publisher

American Chemical Society (ACS)

Subject

Biochemistry

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4. Are we underestimating microplastic abundance in the marine environment? A comparison of microplastic capture with nets of different mesh-size

5. Current understanding of microplastics in the environment: Occurrence, fate, risks, and what we should do

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