Spiked Genes: A Method to Introduce Random Point Nucleotide Mutations Evenly throughout an Entire Gene Using a Complete Set of Spiked Oligonucleotides for the Assembly-Reference-Cited by-同舟云学术

Spiked Genes: A Method to Introduce Random Point Nucleotide Mutations Evenly throughout an Entire Gene Using a Complete Set of Spiked Oligonucleotides for the Assembly

Published:2017-07-05 Issue:7 Volume:2 Page:3183-3191
ISSN:2470-1343
Container-title:ACS Omega
language:en
Short-container-title:ACS Omega

Author:

Cárcamo Edson¹,Roldán-Salgado Abigail¹,Osuna Joel¹,Bello-Sanmartin Iván¹,Yáñez Jorge A.¹,Saab-Rincón Gloria¹,Viadiu Héctor²,Gaytán Paul¹^ORCID

Affiliation:

1. Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Cuernavaca, Morelos 62210, México

2. Instituto de Química, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, Ciudad de Mexico 04510, México

Publisher

American Chemical Society (ACS)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.acs.org/doi/pdf/10.1021/acsomega.7b00508

Reference59 articles.

1. Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution

2. Methods for the directed evolution of proteins

3. Enzyme engineering reaches the boiling point

4. Widening the pH Activity Profile of a Fungal Laccase by Directed Evolution

5. Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis

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