Quick and Easy Assembly of a One-Step qRT-PCR Kit for COVID-19 Diagnostics Using In-House Enzymes

Author:

Takahashi Masateru1,Tehseen Muhammad1,Salunke Rahul2,Takahashi Etsuko1,Mfarrej Sara2,Sobhy Mohamed A.1,Alhamlan Fatimah S.3,Hala Sharif245,Ramos-Mandujano Gerardo6,Al-Qahtani Ahmed A.3,Alofi Fadwa S.7,Alsomali Afrah8,Hashem Anwar M.910,Khogeer Asim11,Almontashiri Naif A. M.1213,Lee Jae Man14,Mon Hiroaki14,Sakashita Kosuke15,Li Mo6,Kusakabe Takahiro14,Pain Arnab2,Hamdan Samir M.1ORCID

Affiliation:

1. Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia

2. Pathogen Genomics Laboratory, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia

3. Department of Infection and Immunity, King Faisal Specialist Hospital and Research Center, Riyadh 11211, Saudi Arabia

4. King Saud Bin Abdulaziz University of Health Sciences, Jeddah 22384, Saudi Arabia

5. King Abdullah International Medical Research Centre, Jeddah, Makkah, Ministry of National Guard Health Affairs, Jeddah, Makkah 22384, Saudi Arabia

6. Stem Cell and Regenration Laboratory. Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia

7. Infectious Diseases Department, King Fahad Hospital, Madinah 3177, Saudi Arabia

8. King Abdullah Medical Complex (KAMC), Jeddah 23816, Saudi Arabia

9. Vaccines and Immunotherapy Unit, King Fahd Medical Research Center; King Abdulaziz University, Jeddah, Saudi Arabia

10. Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia

11. Plan and Research Department, General Directorate of Health Affairs Makkah Region, MOH Mecca 24321, Saudi Arabia

12. College of Applied Medical Sciences, Taibah University, Madinah 41311, Saudi Arabia

13. Center for Genetics and Inherited Diseases, Taibah University, Madinah 42353, Saudi Arabia

14. Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan

15. Department of Infection and Immunity, King Faisal Specialist Hospital and Research Centre, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia

Funder

King Abdullah University of Science and Technology

Publisher

American Chemical Society (ACS)

Subject

General Chemical Engineering,General Chemistry

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