A Sensitive and Robust Enzyme Kinetic Experiment Using Microplates and Fluorogenic Ester Substrates
Author:
Affiliation:
1. Department of Chemistry, Butler University, Indianapolis, Indiana 46208, United States
2. Janelia Farm Research Campus, The Howard Hughes Medical Institute, Ashburn, Virginia 20147, United States
Funder
Division of Undergraduate Education
Howard Hughes Medical Institute
Publisher
American Chemical Society (ACS)
Subject
Education,General Chemistry
Link
https://pubs.acs.org/doi/pdf/10.1021/ed500452f
Reference21 articles.
1. Microplate Bioassay for Determining Substrate Selectivity of Candida rugosa Lipase
2. 96-Well Plate Colorimetric Assay for Ki Determination of (+/-)-2-Benzylsuccinic Acid, an Inhibitor of Carboxypeptidase A: A Laboratory Experiment in Drug Discovery
3. Lactate dehydrogenase kinetics and inhibition using a microplate reader
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