A Complementary Isothermal Amplification Method to the U.S. EPA Quantitative Polymerase Chain Reaction Approach for the Detection of Enterococci in Environmental Waters

Author:

Kolm Claudia12ORCID,Martzy Roland12,Brunner Kurt1,Mach Robert L.3,Krska Rudolf4,Heinze Georg5,Sommer Regina62,Reischer Georg H.13,Farnleitner Andreas H.372ORCID

Affiliation:

1. TU Wien, Institute of Chemical, Environmental & Biological Engineering, Molecular Diagnostics Group, Department IFA-Tulln, Konrad-Lorenz-Straße 20, 3430 Tulln, Austria

2. ICC Interuniversity Cooperation Centre Water & Health, Vienna, Austria (ICC Interuniversity Cooperation Center Water & Health, Vienna, Austria (www.waterandhealth.at)

3. TU Wien, Institute of Chemical, Environmental & Biological Engineering, Research Group of Environmental Microbiology and Molecular Diagnostics 166/5/4, Gumpendorferstraße 1a, 1060 Vienna, Austria

4. University of Natural Resources and Life Sciences, Vienna (BOKU), Department IFA-Tulln, Center for Analytical Chemistry, Konrad-Lorenz-Straße 20, 3430 Tulln, Austria

5. Medical University Vienna, Center for Medical Statistics, Informatics and Intelligent Systems, Section for Clinical Biometrics, Spitalgasse 23, 1090 Vienna, Austria

6. Medical University Vienna, Institute for Hygiene and Applied Immunology, Water Hygiene, Kinderspitalgasse 15, 1090 Vienna, Austria

7. Karl Landsteiner University of Health Sciences, Research Unit Water Quality and Health, Dr.-Karl-Dorrek-Straße 30, 3500 Krems, Austria

Funder

Austrian Science Fund

NÖ Forschungs- und Bildungsges.m.b.H

Publisher

American Chemical Society (ACS)

Subject

Environmental Chemistry,General Chemistry

Reference57 articles.

1. Escherichia coli and enterococci are sensitive and reliable indicators for human, livestock and wildlife faecal pollution in alpine mountainous water resources

2. Water quality – Detection and Enumeration of Intestinal Enterococci – Part 2: Membrane Filtration Method. ISO Technical Report 7899-2:2000;International Organization of Standardization:Geneva, 2000.

3. Method 1600: Enterococci in water by membran filtration using membrane-enterococcus indoxyl-β-d-glucoside agar (mEI). Technical Report EPA-821-R-09-016;Office of Water, U.S. Environmental Protection Agency:Washington, DC, 2009.

4. Quantitative microbial faecal source tracking with sampling guided by hydrological catchment dynamics

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