1. After removing the solvent of the mycelium extract, the residue was triturated withn-BuOH, and then-BuOH layer was concentrated. The residual oil was suspended in tetrahydrofuran, and an active amorphous precipitate was obtained. After this precipitate was washed with a solution of CHCl3/MeOH (2:1), the residue was finally purified by reversed-phase HPLC under basic conditions to afford1in the yield of 12 mg/100 mL.1: HR-FABMS (positive, glycerol matrix)m/z1280.7749 (M + Na)+(calcd for C62H115O24NNa, 1280.7707); FABMS (negative, glycerol matrix)m/z1256 (M − H)-; Anal. Calcd for C62H115O24N.7H2O: C, 53.78; H, 9.39; O, 35.82; N, 1.04. Found: C, 53.96; H, 8.95; O, 35.77; N, 1.08; IR νmax(KBr) (cm-1) 3380, 1600, 1450, 1060; UV λmax(nm) (ε) (MeOH−H2O, 1:1) 299 (6200), 247 (11 000); (MeOH−0.01 N NaOH, 1:1) 299 (6200), 247 (11 000); (MeOH−0.01 N HCl, 1:1) 314 (7300), 237 (7900); [α]19D−2.6° (c0.545, DMSO).
2. Total synthesis of the Fusarium toxin equisetin: proof of the stereochemical relationship of the tetramate and terpenoid sectors
3. Geldanamycin. 3. Biosynthetic origin of the C2 units of geldanamycin and distribution of label from D-[6-13C]glucose