Switching the Ligand Specificity of the Biosensor XylS from meta to para-Toluic Acid through Directed Evolution Exploiting a Dual Selection System-Reference-Cited by-同舟云学术

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Switching the Ligand Specificity of the Biosensor XylS from meta to para-Toluic Acid through Directed Evolution Exploiting a Dual Selection System

Published:2019-11-05 Issue:12 Volume:8 Page:2679-2689
ISSN:2161-5063
Container-title:ACS Synthetic Biology
language:en
Short-container-title:ACS Synth. Biol.

Author:

Ogawa Yuki¹,Katsuyama Yohei¹²,Ueno Kento¹,Ohnishi Yasuo¹²^ORCID

Affiliation:

1. Department of Biotechnology, The Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan

2. Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan

Funder

Japan Society for the Promotion of Science

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

Link

https://pubs.acs.org/doi/pdf/10.1021/acssynbio.9b00237

Reference37 articles.

1. Design, Construction, and Characterization of a Set of Biosensors for Aromatic Compounds

2. Novel screening methods—biosensors

3. High-Throughput Metabolic Engineering: Advances in Small-Molecule Screening and Selection

4. Directed evolution and synthetic biology applications to microbial systems

5. SoxR as a Single-Cell Biosensor for NADPH-Consuming Enzymes in Escherichia coli

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2. Advances in ligand-specific biosensing for structurally similar molecules;Cell Systems;2023-12

3. State-of-the-art in engineering small molecule biosensors and their applications in metabolic engineering;SLAS Technology;2023-10

4. Tunable T7 Promoter Orthogonality on T7RNAP for cis-Aconitate Decarboxylase Evolution via Base Editor and Screening from Itaconic Acid Biosensor;ACS Synthetic Biology;2023-09-26

5. Engineering the Substrate Specificity of Toluene Degrading Enzyme XylM Using Biosensor XylS and Machine Learning;ACS Synthetic Biology;2023-02-03

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