Application of CRISPR-Cas12a Enhanced Fluorescence Assay Coupled with Nucleic Acid Amplification for the Sensitive Detection of African Swine Fever Virus

Author:

Tao Dagang1,Liu Jiajia2,Nie Xiongwei1,Xu Bingrong1,Tran-Thi Thuy-Nhien1,Niu Lili2,Liu Xiangdong1,Ruan Jinxue1,Lan Xiaochen1,Peng Guiqing3,Sun Limeng3,Ma Yunlong14,Li Xinyun14,Li Congcong5,Zhao Shuhong14,Xie Shengsong14ORCID

Affiliation:

1. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, P. R. China

2. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, P. R. China

3. State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan 430070, P. R. China

4. The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P. R. China

5. College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, P. R. China

Funder

Ministry of Education of the People's Republic of China

Ministry of Agriculture of the People's Republic of China

National Natural Science Foundation of China

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

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