CRISPR-Cas Assisted Shotgun Mutagenesis Method for Evolutionary Genome Engineering

Author:

Zhao Ming12,Gao Miaomiao1,Xiong Liangbin13,Liu Yongjun1,Tao Xinyi1,Gao Bei1ORCID,Liu Min1,Wang Feng-Qing1ORCID,Wei Dong-Zhi1

Affiliation:

1. State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

2. Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding, College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, China

3. Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China

Funder

Natural Science Foundation of Shanghai

China Postdoctoral Science Foundation

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

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