Bottom-Up Design: A Modular Golden Gate Assembly Platform of Yeast Plasmids for Simultaneous Secretion and Surface Display of Distinct FAP Fusion Proteins-Reference-Cited by-同舟云学术

Bottom-Up Design: A Modular Golden Gate Assembly Platform of Yeast Plasmids for Simultaneous Secretion and Surface Display of Distinct FAP Fusion Proteins

Published:2022-10-19 Issue:11 Volume:11 Page:3681-3698
ISSN:2161-5063
Container-title:ACS Synthetic Biology
language:en
Short-container-title:ACS Synth. Biol.

Author:

Szent-Gyorgyi Christopher¹,Perkins Lydia A.¹,Schmidt Brigitte F.²,Liu Zhen²^ORCID,Bruchez Marcel P.¹³²^ORCID,van de Weerd Robert¹^ORCID

Affiliation:

1. Molecular Biosensor & Imaging Center (MBIC), Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, United States

2. Department of Chemistry, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, United States

3. Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, United States

Funder

National Institute of General Medical Sciences

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

Link

https://pubs.acs.org/doi/pdf/10.1021/acssynbio.2c00283

Reference53 articles.

1. A One Pot, One Step, Precision Cloning Method with High Throughput Capability

2. Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

3. A Modular Cloning System for Standardized Assembly of Multigene Constructs

4. Genetic Toolkits to Design and Build Mammalian Synthetic Systems

5. Recent Advances in Strategies for the Cloning of Natural Product Biosynthetic Gene Clusters

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