Rapid and Error-Free Site-Directed Mutagenesis by a PCR-Free In Vitro CRISPR/Cas9-Mediated Mutagenic System-Reference-Cited by-同舟云学术

Rapid and Error-Free Site-Directed Mutagenesis by a PCR-Free In Vitro CRISPR/Cas9-Mediated Mutagenic System

Published:2018-08-03 Issue:9 Volume:7 Page:2236-2244
ISSN:2161-5063
Container-title:ACS Synthetic Biology
language:en
Short-container-title:ACS Synth. Biol.

Author:

She Wenwen¹,Ni Jing¹,Shui Ke²,Wang Fei¹,He Ruyi¹,Xue Jinhui¹,Reetz Manfred T.³⁴^ORCID,Li Aitao¹,Ma Lixin¹^ORCID

Affiliation:

1. Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan 434200, China

2. Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China

3. Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Muelheim, Germany

4. Department of Chemistry, Philipps-University, Hans-Meerwein-Strasse 4, 35032 Marburg, Germany

Funder

Hubei Province

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

Link

https://pubs.acs.org/doi/pdf/10.1021/acssynbio.8b00245

Reference51 articles.

1. Synthetic DNA and Biology (Nobel Lecture)

2. Site saturation mutagenesis: Methods and applications in protein engineering

3. Gene Mutagenesis Methods

4. Laboratory Evolution of Stereoselective Enzymes: A Prolific Source of Catalysts for Asymmetric Reactions

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