Rational Design of Single Copy Expression Cassettes in Defined Chromosomal Sites Overcomes Intraclonal Cell-to-Cell Expression Heterogeneity and Ensures Robust Antibody Production-Reference-Cited by-同舟云学术

Rational Design of Single Copy Expression Cassettes in Defined Chromosomal Sites Overcomes Intraclonal Cell-to-Cell Expression Heterogeneity and Ensures Robust Antibody Production

Published:2020-12-31 Issue:1 Volume:10 Page:145-157
ISSN:2161-5063
Container-title:ACS Synthetic Biology
language:en
Short-container-title:ACS Synth. Biol.

Author:

Gödecke Natascha¹,Herrmann Sabrina¹,Hauser Hansjörg²,Mayer-Bartschmid Anke³,Trautwein Mark⁴,Wirth Dagmar¹⁵^ORCID

Affiliation:

1. RG Model Systems for Infection and Immunity, Helmholtz Centre for Infection Research, Braunschweig 38124, Germany

2. Staff Unit Scientific Strategy, Helmholtz Centre for Infection Research, Braunschweig 38124, Germany

3. Biologics Development, Bayer AG, Wuppertal 42113, Germany

4. Biologics Research, Bayer AG, Wuppertal 42113, Germany

5. Institute of Experimental Hematology, Medical University Hannover, Hannover 30625, Germany

Funder

Bayer

Deutsche Forschungsgemeinschaft

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

Link

https://pubs.acs.org/doi/pdf/10.1021/acssynbio.0c00519

Reference79 articles.

1. Illegitimate DNA integration in mammalian cells

2. A massively parallel reporter assay dissects the influence of chromatin structure on cis-regulatory activity

3. High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells

4. Genomics: The amazing complexity of the human transcriptome

Cited by 4 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A precise and sustainable doxycycline-inducible cell line development platform for reliable mammalian cell engineering with gain-of-function mutations;Metabolic Engineering;2024-11

2. A compendium of stable hotspots in the CHO genome;Biotechnology and Bioengineering;2023-04-04

3. Implementation of ubiquitous chromatin opening elements as artificial integration sites for CRISPR/Cas9‐mediated knock‐in in mammalian cells;Engineering in Life Sciences;2023-03-09

4. Use of ubiquitous chromatin opening elements (UCOE) as tools to maintain transgene expression in biotechnology;Computational and Structural Biotechnology Journal;2023