Developing a Versatile Shotgun Cloning Strategy for Single-Vector-Based Multiplex Expression of Short Interfering RNAs (siRNAs) in Mammalian Cells

Author:

Wang Xi12,Yuan Chengfu23,Huang Bo124,Fan Jiaming12,Feng Yixiao25,Li Alexander J.2,Zhang Bo26,Lei Yan25,Ye Zhenyu27,Zhao Ling25,Cao Daigui258,Yang Lijuan26,Wu Di2,Chen Xian29,Liu Bin210,Wagstaff William2,He Fang25,Wu Xiaoxing25,Luo Huaxiu211,Zhang Jing25,Zhang Meng212,Haydon Rex C.2,Luu Hue H.2,Lee Michael J.2,Moriatis Wolf Jennifer2,Huang Ailong13,He Tong-Chuan2ORCID,Zeng Zongyue1213

Affiliation:

1. Ministry of Education Key Laboratory of Diagnostic Medicine, and School of Laboratory and Diagnostic Medicine, Chongqing Medical University, Chongqing, 400016, China

2. Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, Illinois 60637, United States

3. Department of Biochemistry and Molecular Biology, China Three Gorges University School of Medicine, Yichang, 443002, China

4. Department of Clinical Laboratory Medicine, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China

5. The Affiliated Hospitals of Chongqing Medical University, Chongqing, 400016, China

6. Key Laboratory of Orthopaedic Surgery of Gansu Province, and the Departments of Orthopaedic Surgery and Obstetrics and Gynecology, the First and Second Hospitals of Lanzhou University, Lanzhou, 730030, China

7. Department of General Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, 215004, China

8. Department of Orthopaedic Surgery, Chongqing General Hospital, Chongqing, 400013, China

9. Department of Clinical Laboratory Medicine, The Affiliated Hospital of Qingdao University, Qingdao, 266061, China

10. School of Life Sciences, Southwest University, Chongqing, 400715, China

11. Department of Burn and Plastic Surgery, West China Hospital of Sichuan University, Chengdu, 610041, China

12. Department of Orthopaedic Surgery, The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China

13. Key Laboratory of Molecular Biology for Infectious Diseases of The Ministry of Education of China, Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China

Funder

National Center for Advancing Translational Sciences

National Cancer Institute

Ministry of Science and Technology of the People's Republic of China

University of Chicago

U.S. Department of Defense

Mabel Green Myers Research Endowment Fund

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

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