CReasPy-Cloning: A Method for Simultaneous Cloning and Engineering of Megabase-Sized Genomes in Yeast Using the CRISPR-Cas9 System-Reference-Cited by-同舟云学术

CReasPy-Cloning: A Method for Simultaneous Cloning and Engineering of Megabase-Sized Genomes in Yeast Using the CRISPR-Cas9 System

Published:2019-10-30 Issue:11 Volume:8 Page:2547-2557
ISSN:2161-5063
Container-title:ACS Synthetic Biology
language:en
Short-container-title:ACS Synth. Biol.

Author:

Ruiz Estelle¹²,Talenton Vincent¹²,Dubrana Marie-Pierre¹²,Guesdon Gabrielle¹²,Lluch-Senar Maria³⁴,Salin Franck⁵,Sirand-Pugnet Pascal¹²,Arfi Yonathan¹²,Lartigue Carole¹²^ORCID

Affiliation:

1. INRA, UMR 1332 de Biologie du Fruit et Pathologie, F-33140 Villenave d’Ornon, France

2. Univ. Bordeaux, UMR 1332 de Biologie du Fruit et Pathologie, F-33140 Villenave d’Ornon, France

3. EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr Aiguader 88, Barcelona 08003, Spain

4. Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain

5. BIOGECO, INRA, Univ. Bordeaux, 33610 Cestas, France

Funder

Division of Integrative Organismal Systems

Agence Nationale de la Recherche

Horizon 2020 Framework Programme

Publisher

American Chemical Society (ACS)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine

Link

http://pubs.acs.org/doi/pdf/10.1021/acssynbio.9b00224