Complex Dependence of Escherichia coli-based Cell-Free Expression on Sonication Energy During Lysis
Author:
Affiliation:
1. School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive NW, Atlanta, Georgia 30332-0100, United States
Funder
National Institute of Biomedical Imaging and Bioengineering
Publisher
American Chemical Society (ACS)
Subject
Biochemistry, Genetics and Molecular Biology (miscellaneous),Biomedical Engineering,General Medicine
Link
https://pubs.acs.org/doi/pdf/10.1021/acssynbio.3c00312
Reference24 articles.
1. Quantification of Interlaboratory Cell-Free Protein Synthesis Variability
2. Variability in cell-free expression reactions can impact qualitative genetic circuit characterization
3. Characterizing and prototyping genetic networks with cell-free transcription–translation reactions
4. High-throughput preparation methods of crude extract for robust cell-free protein synthesis
5. Methods to reduce variability in E. Coli-based cell-free protein expression experiments
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