Screening of One-Bead-One-Peptide Combinatorial Library Using Red Fluorescent Dyes. Presence of Positive and False Positive Beads

Author:

Marani Mariela M.1,Martínez Ceron María C.1,Giudicessi Silvana L.1,de Oliveira Eliandre1,Côté Simon1,Erra-Balsells Rosa1,Albericio Fernando1,Cascone Osvaldo1,Camperi Silvia A.1

Affiliation:

1. Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, (1113) Ciudad Autónoma de Buenos Aires, Argentina, CIHDECAR-CONICET, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II-Ciudad Universitaria, (1428) Ciudad Autónoma de Buenos Aires, Argentina, Proteomics Platform, Barcelona Science Park, Baldiri Reixac 10, 08028 Barcelona, Spain, Matrix Innovation Inc., 945 Newton...

Publisher

American Chemical Society (ACS)

Subject

General Chemistry

Reference23 articles.

1. The following abbreviations are used: ACN, acetonitrile; AcOH, acetic acid; BSA, bovine serum albumin; BSA-TR, bovine serum albumin-Texas Red; CHCA, α-cyano-4-hydroxycinnamic acid; DCM, dichloromethane; DIPCDI,N,N′-1,3-diisopropylcarbodiimide; DIPEA,N,N-diisopropylethylamine; DMAP, 4-(N,N-dimethylamino)pyridine; DMF,N,N-dimethylformamide; FITC, fluorescein isothiocyanate; Fmoc-amino acids, fluorenylmethoxycarbonylamino acids; HMBA, 4-hydroxymethylbenzoic acid; HOBt, 1-hydroxybenzotriazole; MALDI-TOF MS, matrix-assisted laser desorption ionization time-of-flight mass spectrometry; OBOC, one-bead one-compound; PBS, phosphate-buffered saline; PEG, poly(ethyleneglycol); SA, streptavidin; SA-ATTO, streptavidin ATTO 590 conjugate; SA-POD, streptavidin peroxidase conjugate; SA-TR, streptavidin Texas red conjugate; TBS, Tris buffer saline; TBTU, 1-[bis(dimethylamino)methylene]-1H-benzotriazolium hexafluorophosphateN-oxide; TFA, triflouroacetic acid; THF, tretrahydrofuran; TIS, triisopropylsilane; TOF, time of flight.

2. A new type of synthetic peptide library for identifying ligand-binding activity

3. The chemical synthesis of large random peptide libraries and their use for the discovery of ligands for macromolecular acceptors

4. Combinatorial peptide library methods for immunobiology research

5. From Combinatorial Chemistry to Cancer-Targeting Peptides

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