1. Experimental data: [α]21D+3.9° (c0.10, MeOH); UV λmax(MeOH) 255 nm (ε 11 000);1H NMR (DMSO-d6) δ 1.10 (3H, s, 6-Me), 1.47 (1H, m, H-7e‘), 1.49 (4H, m, H2-5‘ and H2-5‘ ‘), 1.51 (4H, m, H2-6‘ and H2-6‘ ‘), 1.58 (3H, s, H3-19), 1.65 (1H, m, H-7a‘), 1.66 (3H, s, H3-18), 1.67 (3H, s, 9-Me), 1.84 (3H, s, 13-Me), 1.90 (3H, s, 2-Me), 2.04 (1H, m, H-15), 2.14 (1H, m, H-8), 2.27 (1H, m, H-15), 2.94 (1H, m, H-11), 3.10 (4H, m, H2-4‘ and H2-4‘ ‘), 3.28 (4H, m, H2-7‘ and H2-7‘ ‘), 5.07 (1H, t,J= 6.0 Hz, H-16), 5.22 (1H, d,J= 4.2 Hz, H-10), 6.22 (1H, d,J= 15.0 Hz, H-5), 6.33 (1H, d,J= 10.8 Hz, H-12), 6.37 (1H, dd,J= 15.0, 10.8 Hz, H-4), 7.01 (1H, d,J= 10.8 Hz, H-3), 7.20 (8H, br s, 2 × 9‘-NH2and 2 × 9‘ ‘-NH2), 7.76 (2H, br s, H-3‘ and H-3‘ ‘), [8.80 (3H, br s) and 9.01 (1H, br s), 2‘-NH2and 2‘ ‘-NH2], 9.25 (1H, br s, H-8‘b), 9.41 (1H, br s, H-8‘ ‘b), 10.95 (1H, br s, H-1‘a), and 11.06 (1H, br s, H-1‘ ‘a) (a,bmay be interchangeable);13C NMR (DMSO-d6) δ 12.2 (q, 2-Me), 12.3 (q, 13-Me), 17.8 (q, C19), 21.0 (q, 9-Me), 23.8 (q, 6-Me), 24.9 (2C, t, C6‘ and C6‘ ‘), 25.6 (q, C18), 25.6 (2C, t, C5‘ and C5‘ ‘), 30.1 (t, C15), 34.2 (t, C2), 36.1 (d, C8), 38.7 (s, C6), 40.2 (2C, t, C4‘ and C4‘ ‘), 40.5 (2C, t, C7‘ and C7‘ ‘), 44.6 (d, C11), 121.4 (d, C10), 121.7 (d, C16), 122.3 (d, C4), 126.8 (s, C2), 128.1 (s, C13), 132.3 (s, C17), 136.8 (s, C9), 138.7 (d, C3), 142.0 (d, C12), 153.5 (d, C5), 153.5 (2C, s, C9‘ and C9‘ ‘), 156.8 (2C, s, C2‘ and C2‘ ‘), and 169.4 (2C, s, C1 and C14); HMBC cross peaks: H-4/C6; H-10/C8 and 9-Me; H-12/C14 and 13-Me; H-16/C18 and C19; H18/C16, C17, and C19; H-19/C16, C17, and C18; 2-Me/C1, C2, and C3; 6-Me/C5, C6, C7, and C11; 9-Me/C8 and C9; 13-Me/C12, C13, and C14; H2-4‘ and H2-4‘ ‘/C2‘, C5‘, C6‘, C2‘ ‘, C5‘ ‘, and C6‘ ‘; H2-5‘ and H2-5‘ ‘/C6‘ and C6‘ ‘; H2-6‘ and H2-6‘ ‘/C5‘ and C5‘ ‘; H2-7‘ and H2-7‘ ‘/C5‘, C6‘, C9‘, C5‘ ‘, C6‘ ‘, and C9‘ ‘; FABMS (positive, glycerol)m/z681 (M + H)+; HRFABMS (positive, PEG600 in NBA)m/z681.5076 (calcd for C35H61N12O2, Δ +3.6 mmu).