A Synthetic Gene Library Yields a Previously Unknown Glycoside Phosphorylase That Degrades and Assembles Poly-β-1,3-GlcNAc, Completing the Suite of β-Linked GlcNAc Polysaccharides

Author:

Macdonald Spencer S.12,Pereira Jose H.34,Liu Feng2,Tegl Gregor2,DeGiovanni Andy34,Wardman Jacob F.15ORCID,Deutsch Samuel6,Yoshikuni Yasuo6ORCID,Adams Paul D.347,Withers Stephen G.125ORCID

Affiliation:

1. Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, British Columbia V6T 1Z4, Canada

2. Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T 1Z1, Canada

3. Joint BioEnergy Institute, Emeryville, California 94608, United States

4. Molecular Biophysics & Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States

5. Department of Biochemistry & Molecular Biology, University of British Columbia, 2329 West Mall, Vancouver, British Columbia V6T 1Z4, Canada

6. The US Department of Energy Joint Genome Institute, Lawrence Berkley National Laboratory, Berkeley, California 94720, United States

7. Department of Bioengineering, University of California Berkeley, Berkeley, California 94720, United States

Funder

Government of Canada

U.S. Department of Energy

Publisher

American Chemical Society (ACS)

Subject

General Chemical Engineering,General Chemistry

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