Multiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase

Author:

Pfaff Lara1ORCID,Gao Jian23,Li Zhishuai24,Jäckering Anna56ORCID,Weber Gert7ORCID,Mican Jan8ORCID,Chen Yinping9,Dong Weiliang9ORCID,Han Xu23,Feiler Christian G.7,Ao Yu-Fei110,Badenhorst Christoffel P. S.1ORCID,Bednar David811,Palm Gottfried J.12ORCID,Lammers Michael12,Damborsky Jiri811,Strodel Birgit56ORCID,Liu Weidong234ORCID,Bornscheuer Uwe T.1ORCID,Wei Ren1ORCID

Affiliation:

1. Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany

2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West Seventh Avenue, Tianjin Airport Economic Area, Tianjin 300308, China

3. National Technology Innovation Center of Synthetic Biology, 32 West Seventh Avenue, Tianjin Airport Economic Area, Tianjin 300308, China

4. University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049 China

5. Institute of Biological Information Processing: Structural Biochemistry (IBI-7), Forschungszentrum Jülich, Wilhelm-Johnen-Straße, 52428 Jülich, Germany

6. Institute of Theoretical and Computational Chemistry, Heinrich Heine University, Düsseldorf, Universitätsstr. 1, 40225 Düsseldorf, Germany

7. Helmholtz-Zentrum Berlin für Materialien und Energie, Hahn-Meitner-Platz 1, 14109 Berlin, Germany

8. Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5/C13, 625 00 Brno, Czech Republic

9. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China

10. CAS Key Laboratory of Molecular Recognition and Function, Institute of Chemistry, Chinese Academy of Sciences, Zhongguancun North First Street 2, Beijing 100190, China

11. International Clinical Research Center, St. Anne’s University Hospital, Pekarska 53, 656 91 Brno, Czech Republic

12. Department Synthetic and Structural Biochemistry, Institute of Biochemistry, University of Greifswald, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany

Funder

Deutsche Bundesstiftung Umwelt

Ministry of Science and Technology of the People's Republic of China

Ministerstvo Školství, Mládeže a Telovýchovy

China Scholarship Council

National Natural Science Foundation of China

European Commission

Chinese Academy of Sciences

Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project

Publisher

American Chemical Society (ACS)

Subject

Catalysis,General Chemistry

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