Mechanistic Evaluation of the Pros and Cons of Digital RT-LAMP for HIV-1 Viral Load Quantification on a Microfluidic Device and Improved Efficiency via a Two-Step Digital Protocol

Author:

Sun Bing1,Shen Feng,McCalla Stephanie E.1,Kreutz Jason E.,Karymov Mikhail A.1,Ismagilov Rustem F.1

Affiliation:

1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, California 91125, United States

Publisher

American Chemical Society (ACS)

Subject

Analytical Chemistry

Reference26 articles.

1. WHO/UNAIDS/UNICEF. Global HIV/AIDS response: epidemic update and health sector progress towards universal access: progress report 2011,World Health Organization, 2011.http://www.who.int/hiv/pub/progress_report2011/summary_en.pdf.

2. Panel on Antiretroviral Guidelines for Adults and Adolescents. Guidelines for the use of antiretroverial agents in HIV-1-infected adults and adolescents,Department of Health and Human Services, 2012.http://www.aidsinfo.nih.gov/contentfiles/lvguidelines/adultandadolescentgl.pdf. Accessed on 09/15/2012.

3. HIV-1 Viral Load Assays for Resource-Limited Settings

4. Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard?

5. Digital Isothermal Quantification of Nucleic Acids via Simultaneous Chemical Initiation of Recombinase Polymerase Amplification Reactions on SlipChip

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