Abstract
Today, the most accurate way to diagnose animal helminthiasis is laboratory research. Among them, lifelong coproovoscopy methods play a leading role in the study of the spread of gastrointestinal helminthiasis, particularly trichurosis, and in establishing the effectiveness of anthelmintics. Therefore, a promising research direction includes improving and testing modern methods of coproovoscopy for trichurosis in sheep. The work aimed to determine the diagnostic efficiency of the improved flotation method of coproovoscopy for trichurosis in sheep. In laboratory conditions, the effectiveness of well-known flotation methods and the proposed method of coproovoscopy in diagnosing sheep trichurosis were determined. The leading indicators of the effectiveness of laboratory methods were the indicator of the intensity of trichurous invasion, the coagulation ability of the flotation solution, and the crystallization time of a drop of flotation liquid on a glass slide. It was established that all methods of coproovoscopy used in the experiment have flotation properties relative to Trichuris eggs. However, the improved method of coproovoscopy showed a higher flotation ability concerning Trichuris ovis nematode eggs, where the proposed flotation liquid has pronounced coagulation properties concerning undigested feed residues, and also has a slow crystallization time of droplets on a slide, which is more than 50 min. The improved method uses a combined flotation liquid, whose main components were Ca(NO3)2, C12H22O11, and NaCl. The indicator of the intensity of trichuriasis invasion when using the improved method was 67.0 ± 17.5 eggs in 1 g of feces and exceeded the number of trichuriasis eggs detected by flotation methods using NaCl – by 2.3 times (P ˂ 0.001), C12H22O11 – by 2.1 times (P ˂ 0.001), NH4NO3 – 1.3 times (P ˂ 0.01), C12H22O11 + NaCl – 1.1 times. The obtained data on the effectiveness of the improved method of coproovoscopy allows us to recommend it for introduction into production for effective and accurate laboratory lifelong diagnosis of trichurosis in sheep.
Publisher
Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv
Reference21 articles.
1. Alvarado-Villalobos, M. A., Cringoli, G., Maurelli, M. P., Cambou, A., Rinaldi, L., Barbachano-Guerrero, A., Guevara, R., Chapman, C. A., & Serio-Silva, J. C. (2017). Flotation techniques (FLOTAC and mini-FLOTAC) for detecting gastrointestinal parasites in howler monkeys. Parasites & Vectors, 10(1), 586. DOI: https://doi.org/10.1186/s13071-017-2532-7
2. Bowman, D. D., & Lynn, R. C. (2009). Diagnostic parasitology. Georgi’s parasitology for veterinarians. 9th ed. St-Louis, Elsevier.
3. Cebra, C. K., & Stang, B. V. (2008). Comparison of methods to detect gastrointestinal parasites in llamas and alpacas. Journal of the American Veterinary Medical Association, 232(5), 733–741. DOI: https://doi.org/10.2460/javma.232.5.733
4. Cringoli, G. (2004). Coprological diagnosis: what's new? Parassitologia, 46(1-2), 137–139. URL: https://pubmed.ncbi.nlm.nih.gov/15305703
5. Cringoli, G., Rinaldi, L., Veneziano, V., Capelli, G., & Scala, A. (2004). The influence of flotation solution, sample dilution and the choice of McMaster slide area (volume) on the reliability of the McMaster technique in estimating the faecal egg counts of gastrointestinal strongyles and Dicrocoelium dendriticum in sheep. Veterinary Parasitology, 123(1-2), 121–131. DOI: https://doi.org/10.1016/j.vetpar.2004.05.021