Genes resistentes a carbapenémicos y colistina aislados en <i>Musca domestica</i> proveniente de un basural cercano a un hospital de Lima

Abstract

The objective was to determine the presence of genes resistant to carbapenems and plasmid resistance to colistin (mcr-1) in bacteria isolated from Musca domestica in a garbage dump near a hospital in Lima-Peru. Isolation of carbapenemase-producing bacteria was performed in CHROMagar mSuperCARBATM medium and colistin resistance profiling was performed by colistin discs elution method. Detection of blaKPC, blaNDM, blaIMP, blaOXA-48, blaVIM y mcr-1 genes was performed by conventional PCR. Antimicrobial susceptibility profile assessment was performed on the MicroScan automated system. Carbapenemase-producing bacteria were 31/38 and bacteria that were  resistant to colistin were 26/38 with an MIC ≥ 4 μg/mL. Finally, 7 bacterial strains with carbapenem resistance genes (OXA-48 and KPC) and one bacterial strain with plasmid resistance to colistin (mcr-1) were identified.