Substrates and indolbutyric acid in ex vitro rooting of blackberry and raspberry mini-cuttings

Abstract

The objective of this work was to determine the most adequate substrate and indolbutyric acid (IBA) concentration for the ex vitro rooting of the “Xavante” and “Tupy” blackberry and the “Heritage” and “Fall Gold” raspberry mini-cuttings. Explants were transferred to a laminar flow chamber and placed in a MS nutrient medium, giving origin to mini-cuttings whose bases immersed in four different IAC concentrations (0 mg.L-1, 1000 mg.L-1, 2000 mg.L-1, 3000 mg.L-1). Next, they were placed in transparent and articulated Sampack® (10 x 13 x 20) plastic bags with two types of substrates (vermiculite and vermiculite + coconut fiber, in the proportion of 1:1 v/v). For the number of roots variable, vermiculite substrate associated with coconut fiber showed representative results for the Tupy cultivar. However, for the Heritage cultivar, satisfactory results were obtained for the same variable with vermiculite substrate. One point of maximum efficiency was observed with the 1800 mg.L-1 IBA concentration. Results showed that the ex vitro rooting of blackberry and raspberry mini-cuttings may be realized without immersion in an IBA solution. Therefore, the use of vermiculite associated with coconut fibber is the most recommended.