Biochemical studies of blood of experimental rats in assessing acute oral toxicity of Vetom 20.76 based on the predatory fungus <em>Arthrobotrys оligospora</em>

Abstract

The effect of the experimental preparation Vetom 20.76 based on Arthrobotrys oligospora on the biochemical blood parameters of experimental rats was shown. A complex of preclinical studies to study acute toxicity was carried out on nonlinear white rats (females) at eight weeks of age. Four experimental and one control group were formed. Animals from the experimental groups were injected once with the test drug in the range of test doses (5 µl/kg, 50 µl/kg, 300 µl/kg, 5000 µl/kg). The experimental animals were observed daily for 14 days. The animals of the experimental groups had positive growth and development dynamics even at the drug dosage of 5000 µl/kg. A complex of blood biochemical studies was investigated during preclinical studies in experimental animals. The primary biochemical indicators of blood serum studied during the experiment: total protein (g/L), albumin (g/L), globulin (g/L), creatinine (μmol/L), ALT (U/L), glucose (mmol/L), phosphorus (mmol/L), calcium (mmol/L), potassium (mmol/L), sodium (mmol/L) and total cholesterol (mmol/L). At the end of the study, we stated the improvement of indicators characterizing metabolic processes and protective function of the body of experimental rats as a result of using the drug, both relative to the original data and compared with counterparts from the control. During the study, all biochemical indices of the blood serum of the experimental rats in the studied groups remained within the physiological norm, indicating the drug’s safety. Furthermore, no undesirable side effects were recorded in the animals, even when using Vetom 20.76 at the maximum dose. Therefore, Vetom 20.76 was assigned to the fourth toxicity class according to SS (State Standard) 12.1.007-76.