Further researches on the development of trypanosoma gambiense in glossina palpalis

Abstract

The object of these experiments was to try to discover if there is any definite cycle of development of the trypanosome of Sleeping Sickness in the tsetse fly, Glossina palpalis , and if the late or renewed infectivity of the fly coincides with any phase in this development. The mode of experimentation was to feed a cageful of laboratory-bred tsetse flies on an animal whose blood contained numerous trypanosomes, and at the end of various times to kill the flies and examine their intestinal contents. This was done for periods of one day, two days, three days, and so on, up to 56 days. The microscopical examination of preparations made from the intestinal contents on the various days gave information as to the number and appearance of the trypanosomes.