Calcium control of exocytosis and endocytosis in bovine adrenal medullary cells

Abstract

Experimental analysis of the mechanisms of exocytosis and endocytosis has hitherto been hampered by the inaccessibility of the intracellular sites at which they are controlled. We have recently developed a technique that overcomes this problem. Cells are subjected to intense electric fields of brief duration; this renders the plasma membrane permeable without impairing its ability to participate in exocytosis and endocytosis. Working with ‘leaky’ bovine adrenal medullary cells, catecholamine release has a rather specific requirement for Mg-ATP, is activated by micromolar concentrations of ionized Ca and can be inhibited by Mg, detergents, trifluoperazine, high osmotic pressure and various anions. The mechanism of activation by Ca is discussed in some detail.