Abstract
Two chromosomal domains of 80-100 kilobases containingDrosophilachorion genes undergo tissue-specific amplification in ovarian follicle cells during oogenesis. We have investigated the ability of small segments of DNA from within these regions to induce amplification after insertion into new chromosomal sites by P element-mediated transformation. Certain transduced chorion DNA sequences initiated a pattern of tissue-specific differential replication that was identical to norm al chorion amplification. Both the transformed chorion DNA as well as flankingrosyDNA sequences underw ent amplification. O ur results suggest that differential chorion DNA replication is mediated by specific origin-containing sequences located near the centre of the amplified domains. The possible role of such sequences in normal programmes of replication is discussed.
Subject
Industrial and Manufacturing Engineering,General Agricultural and Biological Sciences,General Business, Management and Accounting,Materials Science (miscellaneous),Business and International Management
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