Abstract
Some of the biochemical engineering issues that arise when the purification of proteins is scaled up are examined. The first question addressed is the quantity of various proteins likely to be required. Consideration is given to the order in which isolation procedures may be applied and to the selective removal on a large scale of non-proteinaceous materials. Some general problems such as the effect of mechanical forces on proteins and their complexes and aggregates are examined, together with ways of enhancing the properties of these aggregates and the methods of recovering them. The manner in which on-line process optimization can be achieved during pilot plant trials is discussed and the paper concludes with a summary of new scientific developments to which the biochemical engineer will need to respond.
Subject
Industrial and Manufacturing Engineering,General Agricultural and Biological Sciences,General Business, Management and Accounting,Materials Science (miscellaneous),Business and International Management
Reference19 articles.
1. Precipitation of nucleic acids with polyethyleneimine and the chromotography of nucleic acids and proteins on immobilized polyethyleneimine. Biochim. biophys;Atkinson A.;Acta,1973
2. Affinity flocculation of yeast cell debris by carbohydrate-specific compounds
3. Protein Purification: The Right Step at the Right Time
4. Monomeric insulins obtained by protein engineering and their medical implications
5. Characterisation of an artificial bifunctional enzyme, (3 galactosidase/galactokinase, prepared by gene fusion;Bulow L.;Eur. J. Biochem.,1987
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