Affiliation:
1. Institut de Chimie, Université de NeuchâtelRue Emile-Argand 11, Case postale 2007, Neuchâtel, Switzerland
Abstract
Optically active cobalt(II) complexes are used as reducing agents in the electron-transfer reaction involving horse heart cytochrome
c
. Analysis of the circular dichroism (CD) spectra of reaction products indicates that the corresponding cobalt(III) species of both enantiomers of [Co
II
(alamp)] (H
2
alamp=
N
,
N
′-[(pyridine-2,6-diyl)bis(methylene)]-bis[alanine]) are partly attached to the protein during electron transfer by coordination to an imidazole unit of one of the histidine residues. His-26 and His-33 are both solvent exposed, and the results suggest that one of these histidine residues acts as a bridge in the electron transfer to and from the haem iron of cytochrome
c
. The reaction is enantioselective: the ratio of the relative reactivity at 15 °C is 2.9 in favour of the
R
,
R
-enantiomer. A small induced CD activity in the haem chromophore reveals that some structural changes in the protein occur consecutively with the binding of the cobalt(III) complex.
Subject
Biomedical Engineering,Biochemistry,Biomaterials,Bioengineering,Biophysics,Biotechnology
Cited by
8 articles.
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