Proteomic evidence of dietary sources in ancient dental calculus

Author:

Hendy Jessica12ORCID,Warinner Christina3456ORCID,Bouwman Abigail5,Collins Matthew J.27ORCID,Fiddyment Sarah2ORCID,Fischer Roman8ORCID,Hagan Richard34,Hofman Courtney A.4ORCID,Holst Malin29ORCID,Chaves Eros610,Klaus Lauren46,Larson Greger11ORCID,Mackie Meaghan712ORCID,McGrath Krista2,Mundorff Amy Z.13,Radini Anita2,Rao Huiyun14,Trachsel Christian15,Velsko Irina M.11,Speller Camilla F.216ORCID

Affiliation:

1. Department of Archaeology, Max Planck Institute for the Science of Human History, Jena, Germany

2. BioArCh, Department of Archaeology, University of York, York, UK

3. Department of Archaeogenetics, Max Planck Institute for the Science of Human History, Jena, Germany

4. Laboratories of Molecular Anthropology and Microbiome Research, Department of Anthropology, University of Oklahoma, Norman, USA

5. Institute for Evolutionary Medicine, ETH-Zürich, University of Zürich, Zürich, Switzerland

6. Department of Periodontology, College of Dentistry, University of Oklahoma Health Sciences Center, Oklahoma, OK, USA

7. EvoGenomics, Natural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark

8. Discovery Proteomics Facility, Target Discovery Institute, University of Oxford, Oxford, UK

9. York Osteoarchaeology Ltd, Bishop Wilton, York, UK

10. Pinellas Dental Specialties, Largo, FL 33776, USA

11. The Palaeogenomics and Bio-Archaeology Research Network, Research Laboratory for Archaeology and the History of Art, University of Oxford, Oxford, UK

12. Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

13. Department of Anthropology, College of Arts and Sciences, University of Tennessee, Knoxville, TN, USA

14. Key Laboratory of Vertebrate Evolution and Human Origins of Chinese Academy of Sciences, Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing, People's Republic of China

15. Functional Genomics Center, ETH-Zürich, University of Zürich, Zürich, Switzerland

16. Department of Anthropology, University of British Columbia, Vancouver, BC, Canada

Abstract

Archaeological dental calculus has emerged as a rich source of ancient biomolecules, including proteins. Previous analyses of proteins extracted from ancient dental calculus revealed the presence of the dietary milk protein β-lactoglobulin, providing direct evidence of dairy consumption in the archaeological record. However, the potential for calculus to preserve other food-related proteins has not yet been systematically explored. Here we analyse shotgun metaproteomic data from 100 archaeological dental calculus samples ranging from the Iron Age to the post-medieval period (eighth century BC to nineteenth century AD) in England, as well as 14 dental calculus samples from contemporary dental patients and recently deceased individuals, to characterize the range and extent of dietary proteins preserved in dental calculus. In addition to milk proteins, we detect proteomic evidence of foodstuffs such as cereals and plant products, as well as the digestive enzyme salivary amylase. We discuss the importance of optimized protein extraction methods, data analysis approaches and authentication strategies in the identification of dietary proteins from archaeological dental calculus. This study demonstrates that proteomic approaches can robustly identify foodstuffs in the archaeological record that are typically under-represented due to their poor macroscopic preservation.

Funder

Wellcome Trust

Kennedy Trust Fund

White Rose University Consortium Collaboration Fund

The Max Planck Society

National Science Foundation

DNRF Niels Bohr Professorship

University of York C2D2 Research Priming Fund

Oxford University Fell Fund

Publisher

The Royal Society

Subject

General Agricultural and Biological Sciences,General Environmental Science,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3