Affiliation:
1. Okinawa Institute of Science and Technology Graduate University, Tancha 1919-1, Onna, Okinawa 904-0495, Japan
2. Institute of Life Science, Kurume University, Asahi-machi 67, Kurume, Fukuoka 830-0011, Japan
Abstract
Mitochondria are essential for regulation of cellular respiration, energy production, small molecule metabolism, anti-oxidation and cell ageing, among other things. While the mitochondrial genome contains a small number of protein-coding genes, the great majority of mitochondrial proteins are encoded by chromosomal genes. In the fission yeastSchizosaccharomyces pombe, 770 proteins encoded by chromosomal genes are located in mitochondria. Of these, 195 proteins, many of which are implicated in translation and transport, are absolutely essential for viability. We isolated and characterized eight temperature-sensitive (ts) strains with mutations in essential mitochondrial proteins. Interestingly, they are also sensitive to limited nutrition (glucose and/or nitrogen), producing low-glucose-sensitive and ‘super-housekeeping' phenotypes. They fail to produce colonies under low-glucose conditions at the permissive temperature or lose cell viability under nitrogen starvation at the restrictive temperature. The majority of thesetsmitochondrial mutations may cause defects of gene expression in the mitochondrial genome.mrp4andmrp17are defective in mitochondrial ribosomal proteins.ppr3is defective in rRNA expression, andtrz2andvrs2are defective in tRNA maturation. This study promises potentially large dividends because mitochondrial quiescent functions are vital for human brain and muscle, and also for longevity.
Funder
Ministry of Education, Culture, Sports, Science and Technology
Japan Society for the Promotion of Science
Okinawa Institute of Science and Technology Graduate University
Subject
General Biochemistry, Genetics and Molecular Biology,Immunology,General Neuroscience
Cited by
1 articles.
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