Affiliation:
1. School of Biological Sciences, University of WollongongWollongong, NSW 2522, Australia
2. Department of Zoology, University of GothenburgBox 405, 413 90 Gothenburg, Sweden
Abstract
In the ageing individual, the production of reactive oxygen species (ROS) accelerates with cell senescence. Depending on the heritability of the underlying processes that determine net ROS levels, this may influence ageing
per se
and its evolutionary direction and rate of change. In order to understand the inheritance and evolution of net ROS levels in free-ranging lizards, we used flow cytometry together with ROS-sensitive fluorogenic probes to measure ROS in lizard blood cells. We measured basal levels of (i) non-specific ROS (superoxide, singlet oxygen, H
2
O
2
and peroxynitrite), (ii) superoxide specifically and (iii) superoxide after CCCP treatment, which elevated ROS production in the mitochondria. The cumulative level of non-specific ROS was higher in adults than juveniles and superoxide level showed high heritability and variability among families. We suggest that the evolution of ROS dynamics may be ROS species specific and perhaps depend on the relative degree of uni- or biparental inheritance of ROS main regulatory pathways.
Subject
General Agricultural and Biological Sciences,Agricultural and Biological Sciences (miscellaneous)
Cited by
48 articles.
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