Affiliation:
1. BioMediTech Institute and Department of Signal Processing, Tampere University of Technology, PO Box 553 33101, Tampere, Finland
Abstract
Many genes are spaced closely, allowing coordination without explicit control through shared regulatory elements and molecular interactions. We study the dynamics of a stochastic model of a gene-pair in a head-to-head configuration, sharing promoter elements, which accounts for the rate-limiting steps in transcription initiation. We find that only in specific regions of the parameter space of the rate-limiting steps is orderly coexpression exhibited, suggesting that successful cooperation between closely spaced genes requires the coevolution of compatible rate-limiting step configuration. The model predictions are validated using
in vivo
single-cell, single-RNA measurements of the dynamics of pairs of genes sharing promoter elements. Our results suggest that, in
E. coli
, the kinetics of the rate-limiting steps in active transcription can play a central role in shaping the dynamics of gene-pairs sharing promoter elements.
Funder
Tampere University of Technology President's Graduate Programme
Alfred Kordelinin Säätiö
Suomen Akatemia
Jane ja Aatos Erkon Säätiö
Vilho, Yrjo and Kalle Vaisala Foundation
Subject
Biomedical Engineering,Biochemistry,Biomaterials,Bioengineering,Biophysics,Biotechnology
Cited by
4 articles.
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