Solid state n.m.r. of biopolymers

Abstract

Some aspects of high-resolution 13 C n.m .r. of solid biopolymers are described. Spectra of amino acids, peptides, proteins and DNA are shown. Differences between solution spectra and solid state spectra of a molecule include multiple lines due to multiple crystal forms, splittings of substituted rings and asymmetric splittings of carbons bonded to nitrogen. The inability of magic angle sample spinning to remove 13 C - 14 N dipolar couplings is a consequence of the 14 N quadrupole moment’s altering the angular dependence of the interactions. Solid state n.m .r. of filamentous viruses is given as an example of new biological applications of this type of spectroscopy.