Abstract
By using the patch–clamp technique the effect of 2-decenoic acid (DA) on Ca
2+
-activated potassium (K
+
) channels in the membrane of smooth muscle cells from the human aorta was studied. In the presence of 0.5 μM Ca
2+
and 2 mM Mg
2+
on the cytoplasmic side of the membrane, a more than tenfold elevation in the probability of the channels being open (
p
o
) was observed under the effect of DA. With divalent cation concentrations of less than 1 nM DA caused a more than twofold elevation in
p
o
. In the DA-treated membranes Mg
2+
ions, which normally fail to activate the channels, brought about a nearly threefold increase in the channel activity when applied to the inner membrane surface. Channel sensitivity to the activating effect of cytoplasmic Ca
2+
ions did not increase with the application of DA. Single-channel conductance was unchanged by DA exposure. We suggest that DA alters the Ca
2+
-binding mechanism of the channel, increasing its sensitivity to Mg
2+
ions, presumably owing to membrane fluidization.
Reference20 articles.
1. Properties of single calcium-activated potassium channels in cultured rat muscle
2. Comparison of Ca2+-dependent K+ channels in the membrane of smooth muscle cells isolated from adult and foetal human aorta
3. Action of quinine on Ca 2+- activated K+channels in smooth muscle cell membrane of human aorta. (In Russian.);Bregestovski P. D.;Biol. Membr.,1986
4. Calcium-activated potassium channel with high conductance in cultured smooth muscle cells membrane of human aorta. (In Russian.);Bregestovski P. D.;Biol. Membr.,1985
5. Comparison of vascular smooth muscle cells from adult human, monkey and rabbit in primary culture and in subculture
Cited by
39 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献