Patterns of pair formation and mating in an ectoparasitic caligid copepod Lepeophtheirus salmonis (Krøyer 1837): implications for its sensory and mating biology

Abstract

Non–invasive observations of single cohort and manipulated populations of the sealouse on laboratory–maintained salmon established the sequence of reproductive events and mating. Protandry occurs with adult male emergence synchronized with pre–adult II female emergence, the stage at which most pair formation and pre–copular guarding takes place. Male competence for pair formation and mating was achieved within 24 h of the final moult and pairing occurred according to the preference hierarchy, virgin adult females greater than pre–adult II females much greater than pre–adult I females. This hierarchy broke down when the adult male to unmated female ratio increased rapidly. Males mated successfully not only with newly moulted adult females, but also with older virgin females in which enlargement of the genital complex and premature extrusion of egg strings had occurred. Multiple mating by adult males was demonstrated and may be widespread. Significant inter–host transfer was also demonstrated. Adult males were more mobile than adult females and showed significantly more inter–host transfer. Ablation of the distal tip of the antennules significantly reduced the success of host–finding, pair formation and males' mating.