Release of acetylcholinesterase into the perfusate from the ox adrenal gland

Abstract

Increasing the potassium ion concentration of Tyrode’s solution perfusing an isolated ox adrenal gland leads to a release of acetylcholinesterase activity into the perfusate. The amount of enzyme activity released by added potassium is much reduced if calcium ions are omitted from the perfusion fluid. The activity of acetylcholinesterase secreted into the perfusate was confined to a single isoenzyme which is identical with the only soluble isoenzyme present in splanchnic nerve trunks and with one of the five soluble isoenzymes in the adrenal medulla.