Thermochemical studies of human red blood cells: enthalpy changes on dilution in buffers

Author:

Abstract

Enthalpy changes on dilution of human red blood cells with buffer solutions over the pH range 6 to 8 at 30°C have been measured by microcalorimetry. From the results a value has been obtained for the enthalpy of ionization of the histidine residues in intracellular haemoglobin which is close to the enthalpy of ionization of the imidazole group of histidine in free solution. It has been found that significant enthalpy changes occur when red cell suspensions are diluted with their supernatant liquid which may be indirectly attributed to increased ion concentrations in the interacting diffuse double layers surrounding the red cells in relatively concentrated suspensions.

Publisher

The Royal Society

Subject

General Medicine

Reference8 articles.

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Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. A microcalorimetric study of human erythrocytes in stirred buffer suspensions;Thermochimica Acta;1992-08

2. In Vitro Thermal Studies of Blood Cells;Thermal and Energetic Studies of Cellular Biological Systems;1987

3. Energy changes and muscular contraction.;Physiological Reviews;1978-07

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