Optical projection tomography implemented for accessibility and low cost ( OPTImAL )

Author:

Darling C.1,Kumar S.12,Alexandrov Y.12,de Faye J.3,Almagro Santiago J.3,Rýdlová A.4,Bugeon L.4,Dallman M. J.4,Behrens A. J.35,French P. M. W12,McGinty J.12

Affiliation:

1. Physics Department, Imperial College London , London SW7 2AZ, UK

2. Francis Crick Institute , London NW1 1AT, UK

3. Cancer Stem Cell Laboratory, Institute of Cancer Research , London SW7 3RP, UK

4. Department of Life Sciences, Imperial College London , London SW7 2AZ, UK

5. CRUK Convergence Science Centre & Division of Cancer, Department of Surgery and Cancer, Imperial College , London, UK

Abstract

Optical projection tomography (OPT) is a three-dimensional mesoscopic imaging modality that can use absorption or fluorescence contrast, and is widely applied to fixed and live samples in the mm–cm scale. For fluorescence OPT, we present OPT implemented for accessibility and low cost, an open-source research-grade implementation of modular OPT hardware and software that has been designed to be widely accessible by using low-cost components, including light-emitting diode (LED) excitation and cooled complementary metal-oxide-semiconductor (CMOS) cameras. Both the hardware and software are modular and flexible in their implementation, enabling rapid switching between sample size scales and supporting compressive sensing to reconstruct images from undersampled sparse OPT data, e.g. to facilitate rapid imaging with low photobleaching/phototoxicity. We also explore a simple implementation of focal scanning OPT to achieve higher resolution, which entails the use of a fan-beam geometry reconstruction method to account for variation in magnification. This article is part of the Theo Murphy meeting issue 'Open, reproducible hardware for microscopy'.

Funder

Wellcome Trust

EPSRC National Productivity Investment Fund

Publisher

The Royal Society

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Open, reproducible hardware for microscopy;Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences;2024-06-03

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